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в эпителиальных клетках являются белки цитоскелета (у человека
- цитокератин). /2335к/96/
Высказывались предположения о возможной патогенетической ро-
ли аутоантител, реагирующих с эпителием тимуса, в развитии по-
ражения тимуса и последующем формировании Т-клеточного ИД-та.
Однако прямые данные о последствиях их взаимодействия с эпите-
лиальными клетками тимуса в литературе отсутствуют, что делает
все суждения относительно их физиологической и (или) патогене-
тической роли умозрительными. /2335к/96/
.
Montgomery RA. Dallman MJ.
Semi-quantitative polymerase chain reaction analysis of cytokine and
cytokine receptor gene expression during thymic ontogeny.
Source
Cytokine. 9(10):717-26, 1997 Oct.
Semi-quantitative, polymerase chain reaction (PCR) is used to uncover the
patterns of cytokine transcription in the mouse thymus from day 14 to day
20 of gestation, a time period which includes many of the important events
in thymic ontogeny. Interleukin 4 (IL-4), IL-7 and interferon gamma
(IFN-gamma) mRNA is abundant from fetal day (Fd) 14-16, corresponding with
the period of rapid proliferation of immature thymocytes in vivo. As the
level of mRNA for these cytokines diminishes, the induction and increased
expression of IL-3 and IL-2 occurs. The transcription of these cytokines
correlates temporally with the period of proliferation-dependent
phenotypic differentiation between Fd 16 and 20. The thymic epithelium
(TE)-derived cytokines including IL-1alpha, IL-6 and
granulocyte-macrophage colony-stimulating factor (GM-CSF) begin to be
transcribed between Fd 14-15 and show peak mRNA abundance from Fd 16-20.
IL-5, tumour necrosis factor alpha (TNF-alpha) and LT (lymphotoxin or
TNF-beta) constitute a fourth group of cytokines, along with the IL-4
receptor (IL-4R), which are transcribed at an even level throughout the
fetal period. The IL-2 receptor beta chain (IL-2Rbeta) and IL-10 show
abundant mRNA from Fd 14-20 and have a peak level of mRNA content on Fd
16. Taken together, these studies uncover complex, overlapping patterns of
cytokine gene expression. The mRNA abundance and pattern of expression of
each cytokine or cytokine receptor may indicate the relative contribution
that it makes to different stages of fetal thymic ontogeny.Copyright 1997
Реферат опубликован: 16/06/2005 (6029 прочтено)