Клетки иммунной сисемы. Иммунокомпетентные клетки

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separated as consequences of partial T cell signaling.

Weismann M. Guse AH. Sorokin L. Broker B. Frieser M. Hallmann R.

Mayr GW.

Integrin-mediated intracellular Ca2+ signaling in Jurkat T lymphocytes.

Journal of Immunology. 158(4):1618-27, 1997 Feb 15.

T lymphocytes interact with components of the extracellular matrix after

transendothelial migration on their way to sites of inflammation. To

characterize the molecular basis of the interaction between T lymphocytes

with different extracellular matrix proteins, we investigated the role of

intracellular Ca2+ as a signal mediating such interactions and identified

the cell surface integrins involved in this process. When Jurkat T

lymphocytes loaded with the calcium-sensitive fluorescent dye fura-2 were

placed on coverslips coated with human fibronectin, human collagen types

I, IV, and VI, human tenascin, human laminin I, or mouse laminin I, an

elevation in intracellular Ca2+ concentration was observed. In contrast,

contact of the Jurkat T lymphocytes with vitronectin and thrombospondin

did not induce Ca2+ signals in more cells as compared with control

measurements in which cells were in contact with only BSA or polylysine.

Furthermore, the percentage of Jurkat T lymphocytes responding with Ca2+

signals to collagen types I and IV, fibronectin, and laminin I was

completely reduced to levels observed on BSA or polylysine when the cells

were pretreated with specific anti-integrin Abs, suggesting a role for

cell surface integrins as mediators of cell matrix-induced intracellular

Ca2+ signaling. Similar results were obtained with peripheral human T

lymphocytes activated by phytohemagglutinin.

Реферат опубликован: 18/04/2005 (6426 прочтено)